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cultrex reduced growth factor basement membrane extract type 2  (Trevigen)

 
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    Structured Review

    Trevigen cultrex reduced growth factor basement membrane extract type 2
    Cultrex Reduced Growth Factor Basement Membrane Extract Type 2, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cultrex+basement+membrane+extract/basement+extract+membrane/pm42277016-408-10-20
    Average 86 stars, based on 1 article reviews
    cultrex reduced growth factor basement membrane extract type 2 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Microscopy:

    Article Title: A high-throughput zebrafish screen identifies novel candidate treatments for kaposiform lymphangiomatosis (KLA).
    Article Snippet: Kaposiform lymphangiomatosis (KLA) is a rare and aggressive disease caused by a somatic activating NRAS mutation (p.Q61R) in lymphatic endothelial cells (LECs).. The development of new therapeutic avenues is hampered by the lack of animal models faithfully replicating the clinical manifestations of KLA.. Here, we established a novel zebrafish model of KLA by conditionally expressing the human NRAS mutation in venous and lymphatic ECs.

    Membrane:

    Article Title: A high-throughput zebrafish screen identifies novel candidate treatments for kaposiform lymphangiomatosis (KLA).
    Article Snippet: Kaposiform lymphangiomatosis (KLA) is a rare and aggressive disease caused by a somatic activating NRAS mutation (p.Q61R) in lymphatic endothelial cells (LECs).. The development of new therapeutic avenues is hampered by the lack of animal models faithfully replicating the clinical manifestations of KLA.. Here, we established a novel zebrafish model of KLA by conditionally expressing the human NRAS mutation in venous and lymphatic ECs.

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Article Title: Multi-omics analysis of TNBC organoids identifies phosphorylation of the membrane trafficking machinery as key event associated with FER-mediated invasion
    Article Snippet: After selection, organoids were treated with Doxycycline (Cat# D9891, Sigma-Aldrich) (2 μg/mL) for 3 days and knockdown efficiency was assessed by immunoblotting analysis. .. PDXOs were collected from Cultrex Basement Membrane Extract (BME; 3533-005-02, Trevigen) or Matrigel using 6 mg/mL Dispase (Gibco, 17105041) for 15 minutes, at 37°C. ..

    Article Title: Recruitment of transcriptional effectors by Cas9 creates cis -regulatory elements and demonstrates distance-dependent transcriptional regulation
    Article Snippet: .. The beating monolayers (30 days of differentiation) were enzymatically dissociated into small clusters or single cardiomyocytes using TrypLE and then plated on Cultrex Basement Membrane Extract (BME; Trevigen) coated wells. ..

    Article Title: Recruitment of transcriptional effectors by Cas9 creates cis regulatory elements and demonstrates distance-dependent transcriptional regulation
    Article Snippet: Cardiomyocyte fraction was purified by density 586 centrifugation in Percoll (Sigma-Aldrich, St. Louis, MO) gradient. .. Cardiomyocytes were plated on dishes 587 coated with Cultrex Basement Membrane Extract (BME; Trevigen) diluted with serum-free DMEM. ..

    Article Title: MiRNAs profile of extracellular vesicles from differentiated endothelial cells treated with chemical environmental pollutant BPA
    Article Snippet: .. To study the capillary-like tube structure formation ability, differentiated cells were cultured in 12-well culture plates, treated with 300 μl/ well of Cultrex® Basement Membrane Extract (Trevigen, Inc., Gaithersburg, MD, USA) and observed at an inverted light microscope after 1 h of culture. ..

    Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modeling
    Article Snippet: PureCol , Advanced BioMatrix , Cat#5005. .. Cultrex Basement Membrane Extract, Type 2 , Trevigen , Cat#3532-010. .. TrypLE express enzyme , Thermo Fisher Scientific , Cat#12605010.

    Article Title: Recruitment of transcriptional effectors by Cas9 creates cis -regulatory elements and demonstrates distance-dependent transcriptional regulation
    Article Snippet: Cardiomyocyte fraction was purified by density centrifugation in Percoll (Sigma-Aldrich, St. Louis, MO) gradient. .. Cardiomyocytes were plated on dishes coated with Cultrex Basement Membrane Extract (BME; Trevigen) diluted with serum-free DMEM. ..

    Protein Concentration:

    Article Title: A high-throughput zebrafish screen identifies novel candidate treatments for kaposiform lymphangiomatosis (KLA).
    Article Snippet: Kaposiform lymphangiomatosis (KLA) is a rare and aggressive disease caused by a somatic activating NRAS mutation (p.Q61R) in lymphatic endothelial cells (LECs).. The development of new therapeutic avenues is hampered by the lack of animal models faithfully replicating the clinical manifestations of KLA.. Here, we established a novel zebrafish model of KLA by conditionally expressing the human NRAS mutation in venous and lymphatic ECs.

    Stable Transfection:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Transfection:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Cell Culture:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Article Title: MiRNAs profile of extracellular vesicles from differentiated endothelial cells treated with chemical environmental pollutant BPA
    Article Snippet: .. To study the capillary-like tube structure formation ability, differentiated cells were cultured in 12-well culture plates, treated with 300 μl/ well of Cultrex® Basement Membrane Extract (Trevigen, Inc., Gaithersburg, MD, USA) and observed at an inverted light microscope after 1 h of culture. ..

    Inhibition:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Incubation:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Centrifugation:

    Article Title: Irreversible inhibitors of c-Src kinase that target a non-conserved cysteine
    Article Snippet: .. A. NIH-3T3 cells stably transfected with v-Src in 3D cell culture growth inhibition: 3D Cell Culture Procedure: Prior to plating cells, 50 μL Cultrex basement membrane extract (BME, Trevigen) was added to each well of a 96-well plate incubated on ice and then allowed to gel (cushion formation) over a 30-minute time period in a 37oC incubator. v-Src 3T3 cells were then dispersed from flasks and collected by centrifugation (200xg for 5 minutes at room temperature). ..

    Light Microscopy:

    Article Title: MiRNAs profile of extracellular vesicles from differentiated endothelial cells treated with chemical environmental pollutant BPA
    Article Snippet: .. To study the capillary-like tube structure formation ability, differentiated cells were cultured in 12-well culture plates, treated with 300 μl/ well of Cultrex® Basement Membrane Extract (Trevigen, Inc., Gaithersburg, MD, USA) and observed at an inverted light microscope after 1 h of culture. ..



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